Validation of short run time GC-EI (+) MS/MS method for pesticide residues determination in dry Hibiscus syriacus L. via application of d-MRM and end-point backflush quantification techniques

In this paper, the short injection method using GC-EI (+) MS/MS with end-point back-flush and dynamic-multi-reaction mechanism technique was validated for simultaneous determination of pesticide residues in dry Hibiscus syriacus L. applying QuEChERS extraction covering 176 GC-amenable residues. The GC optimum temperature program achieved a run time it 20 minutes. linearity evaluated matrix matched calibration standards at five concentration levels results showed that coefficient (r2) each analyte more than 0.990. LODs considered to be lowest could detected under optimal experimental conditions 2 μg/kg (S/N = 3) LOQs 10 10). Acceptable recoveries all selected pesticides three fortification were range from 60% 102% relative standard deviations ≤20% (n 6) effect ranged −31% 6%‎. expanded measurement uncertainty estimated as 40% (coverage factor k 2, confidence level 95%). validation prove fit intended use accordance EC guidelines (SANTE/12682/2019) successfully expand detection efficiency other commodities.